AMETEC Journal

Workshop on "“Biochemical responses of fish to oil spills and other marine pollutants”

A N N O U N C E M E N T

APEC Marine Environmental Training and Educational Center
South Sea Research Institute,
Korea Ocean Research and Development Institute
Geoje, Korea


APEC-AMETEC sponsored workshop on

“Biochemical responses of fish to oil spills and other marine pollutants”

Date: November 3-14, 2008
Venue: SSRI, KORDI, Geoje, Korea

Instructed by experts from Australia, Canada, Korea and the USA


Following an oil spill or other cases of marine pollution, evidence is often required to demonstrate the “zone of influence” or the severity of the event. Biochemical responses of fish are increasingly being used in such assessments; these include measurement of the concentration and/or the enzymatic activity of Cytochrome P-450 1A (CYP1A) both of which may be induced in fish liver by exposure of the fish to polycyclic aromatic hydrocarbons (PAH). In this course, the following methods will be taught:

o Measurement of hepatic ethoxyresorufin-O-de-ethylase (“EROD”) (an indicator of the catalytic activity of CYP1A) by fluorescence spectrophotometry;
o Measurement of PAH metabolite concentrations in bile by fluorescence spectroscopy;
o Measurement of CYP1A concentrations by immunochemical methods.

The course will be taught by

Dr. R.F. Addison (R.F. Addison Environmental Consulting, Ltd., Canada),
Dr. T.K. Collier (NOAA, USA),
Dr. J.H. Jung (KORDI, Korea) and
Dr. S. Codi King (AIMS, Australia),


all of whom are experienced in using these approaches during or following oil spills. The course will consist of lectures and practical laboratory work.

The course will be held at KORDI (Korea Ocean Research and Development Institute) South Sea Research Institute, Geoje Island, South Korea, from November 3 - 14 (inclusive) 2008.

10 participants from 9 APEC economies with qualifications of minimum B.Sc. in Chemistry/Biology (most of them have either a M.Sc. or doctoral research training - doctoral students) have been selected.

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